Composite

Part:BBa_K209449:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)


pEF1-SSFYFP-hM2D-LPD(775-1250)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 346
    Illegal PstI site found at 2635
    Illegal PstI site found at 3010
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1161
    Illegal PstI site found at 346
    Illegal PstI site found at 2635
    Illegal PstI site found at 3010
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
    Illegal BamHI site found at 955
    Illegal BamHI site found at 1665
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 346
    Illegal PstI site found at 2635
    Illegal PstI site found at 3010
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 346
    Illegal PstI site found at 2635
    Illegal PstI site found at 3010
    Illegal AgeI site found at 2516
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal BsaI site found at 2980
    Illegal BsaI.rc site found at 2927
    Illegal BsaI.rc site found at 2994
    Illegal BsaI.rc site found at 3667


Design Notes

Cloned into pEF5/FRT/V5-DEST.


Source

References